The PLRP 2010 field season has come to a close and I am both saddened by the fact that operations are finished for another year but excited by the prospect of adding the data we’ve collected this year to our growing body of knowledge about this unique lake. I am in awe of the work that has been done by this amazing team and of how much we’ve grown, while maintaining the sense of adventure and camaraderie that to me, helps to define the PLRP.

We’ve taken great strides towards answering many of our research questions and in the process, with every answer we have come up with many more questions that will keep the PLRP team occupied for quite some time. Fortunately, our family continues to grow and every year we welcome new individuals who bring a unique perspective and desire to tease out the mysteries Pavilion has to offer. We have also been blessed this year by the addition of two little members to the PLRP family, Darlene Lim’s daughter Amelia and Greg Slater’s son Joseph. We look forward to the day when they are exploring the lake alongside us.

DeepWorker Pilots and Nuytco Team: 2010

The PLRP provides a wealth of research opportunities, and not just those focused on understanding the processes leading to the formation of the structures at Pavilion Lake but also to understanding fundamental biological, chemical and physical processes. The research contributions from our participating scientists and graduate students have resulted in a number of recent publications and are essential to increasing our understanding of Earth and astrobiological systems. We’re very proud of the role that the PLRP has played in developing operational technologies and protocols that not only help us meet our science objectives but provide important input into future space science missions.

With the addition of our two newest scientist pilots, astronauts Chris Hadfield and Stan Love, we had 34 DeepWorker missions over 10 days of operations. This year we were aided greatly in our pre-season flight planning by the wonderful team from NASA Ames led by Matt Deans and David Lees who developed an amazing flight planning tool that enabled us to search images and flight paths from previous years while building flight plans in Google Earth. Flights this year were planned to collect images of the remaining unexplored regions of the lake, to record detailed images of areas of interest identified from 2008 and 2009 data and to use the submersibles in combination with other analytical tools such as a conductivity, temperature and depth (CTD) instrument and our autonomous underwater vehicle(s) (AUV). Our ability to review post-flight video data in the field, an effort pioneered in 2009, added greatly to our productivity as this information was used immediately by the science backroom team to modify existing flight plans to best optimize our data collection. As part of the daily flight debriefs, we have also continued to apply metrics associated with scientific productivity to understand factors that influence scientific exploration. New this year to the DeepWorker flight repertoire were long ~ 5 hour flights and two night flights to investigate the grazing activity that we suspect may occur in the lake. To add to the innovations this year, Nick Wilkinson designed a fantastic, interactive program for use in classifying the microbialite images. This new tool will allow us to efficiently organize and process our field data over the coming months. Stay tuned for updates.

The Amazing Pavilion Lake Research Project Team: 2010!

In case our DeepWorker operations didn’t keep us busy enough, we had a number of other important activities included in the field schedule this year. The UBC and University of Delaware AUV teams produced fantastic images of the lake bottom that were often used to compliment the DeepWorker flights and give us a better picture of where interesting structures and features are in the lake. Numerous SCUBA dives were performed by our intrepid team of divers to collect water and microbialite samples that were shared between various research groups in an effort to combine and compliment analytical findings. These samples will be characterized from a virology, microbial lipid, isotopic and genetic point of view to provide more information about the role of biology in the formation of the microbialites and what biosignatures may be left behind. Water samples were collected from nearby lakes including Crown, Turquoise, Pear and Kelly Lake to continue to help us put Pavilion Lake in context. Kelly Lake, which also hosts microbialites and has been an area of interest to the PLRP team for many years, was also the focus of significant AUV activities this year. Microbial mats were once again collected from the Cariboo Plateau lakes and giant pancakes were eaten by all (well, almost all). As a new participatory activity this year, our visiting teacherswere given the task of selecting a SCUBA dive based on their understanding of the research questions of interest (on their first day no less!). I’m happy to report that they eagerly interviewed members of the team before presenting their selected dive and rationale to the group for inclusion in the next day’s diving schedule. Community Day was another great success this year with the team happy to show off our work and answer questions from the many visitors we had to the site. Busy indeed!

We plan on continuing our updates throughout the year as we analyze samples and work through the amazing amount of data that were collected. Thanks to all who have read about our activities and through this process, have joined in our adventure. See you next year!

~ Allyson

Today was my first research dive of the 2010 PLRP season, starting a project on microbialite morphology. I am a geology graduate student from UC-Davis and, while I’m not a true microbiologist, my thesis work is focused on the potential signatures that their communities can leave in the rock record.

Side view of the MOUS showing vertically oriented growth structures (note lasers are 10cm apart)

Growth processes in the Pavilion Lake microbialites may give insight into the significance of ancient microbial carbonates. I’m particularly excited to join the PLRP crew because of the wide range of microbialite morphologies that are present here. In the course of the next two weeks, I will be diving on one particularly large microbialite, affectionately dubbed the MOUS (microbialite of unusual size). The carbonate structure is apparently templating a boulder from a rockslide. While today was mostly an exploratory dive to photograph and survey the structure, I will mostly be investigating the relationship among light regime, microbialite morphology, and invertebrate grazers.

Above the MOUS with blocks of microbialite

Locating the MOUS underwater was our first task of the day. We dropped down near its recorded location and then followed the lake bottom down along a landmark rockslide until we reached 85 feet. From previous dive records, we knew that the top of the structure was at 87 feet, so we swam parallel to shore until we intersected it. The visibility in Pavilion Lake is great (particularly as I’ve done most of my training off of Northern CA) so it was pretty easy to spot.

Sediment deposit with surrounding growth on the top of the MOUS

While my dive buddy, Mike Delaney, worked putting in a temporary transect line to help us more reliably locate the structure (particularly during night dives), I photographed some of the major regions. Large blocks have spalled off the side of the structure throughout its growth, forming an incipient conglomerate of sorts at its base. I’d love to see this in the rock record!

Mike Delaney installing temporary transect line

One of the aspects of modern analogues that really fascinates me is time-averaging. What we see here on the surface of the lake is a geological instant, and over time the current growth surface will be incorporated into the microbialite subsurface. What would this look like? Outside of this project, one of my broader research questions is determining what sort of paeleoenvironmental record might be left in a microbialite, and how that signature is altered with preservation, or lack thereof.

I’m excited to learn more about the interactions between these microbialite structures and their surrounding environment as the field season progresses. There is always room for the unexpected in fieldwork, and I look forward to seeing what future dives will uncover in the lives of these microbial communities.

-Tyler

Editor’s note: Tyler’s boyish good looks have earned him the affectionate nickname “Boy” among the science team – resulting in the title of the blog entry.

The day before submersible operations is always rife with anticipation. Our whole team has been working all year to prepare for this mission, so now that we are so close to beginning, it can feel a bit torturous to wait one more day! However, there is lots of work and science to fill today. I spent some of my time today doing my first scuba dives of the field season. To make sure that everyone is safe, we do scuba checkout dives to practice our skills. After completing those, I headed off with a small team to the south basin of Pavilion Lake. We piled into a zodiac (a small, fast, inflatable boat) with our gear, and enjoyed the 10 minute ride south. Alex and Bernard jumped off, and swam over to place some sensors in one of the springs that we have discovered. Mike and I split off to investigate and collect samples of some of the microbial mats that inhabit the shallow waters of the lake.

Bekah preparing for a dive at Pavilion Lake

The mats are exciting for me because I am interested in the way some of the bacterial cells (specifically Cyanobacteria) move. The movement patterns of these single celled organisms can create complex shapes, or morphologies visible to the naked eye. Microbialites are “organosedimentary structures”, which just means that they are structures built out of microbial mats and minerals or sediments. The soft mats that I am studying are building complex structures, but they are not involving lots of minerals and sediments (they aren’t actually forming microbialites, so you might wonder about my interest). However, because the mats form complex morphologies, many of the bacterial behaviors that we observe in the soft mats will help us understand the formation and morphogenesis of other microbialites.

Studying these soft microbial mats involves lots of photographic documentation, as well as collecting samples for studying in the lab. We can actually grow these mats in the lab, and watch how the movement patterns work to build complex shapes. I work underwater with a pair of tweezers and carefully collect small pieces of mat into small plastic tubes. I carefully transport them back to the lab on shore, where we can begin our experiments. It doesn’t look very exciting underwater because I spend lots of time in the same place, but I actually really enjoy it! The mats are fascinating, and working underwater is very relaxing. Submersibles are fun, but sometimes it is nice to get your face right up close to what you are studying. They are both great ways to do science!

-Bekah