Posts Tagged ‘Microbialites’

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Wednesday, July 15th, 2009

Tracking submarines on the go!

by Matthew Deans

For the past 10 days we have been making real time maps of all of the sub operations at Pavilion Lake. To do this, we have written some software to generate KML files to load into Google Earth. The Intelligent Robotics Group at NASA Ames has a lot of experience with ground data systems for NASA missions like the MER rovers, robotic earth analog field tests like the Robotic Recon test in northern Arizona in June, disaster response applications, and the Gigapan camera system, we were able to put together a set of tools to support sub tracking and mapping using a lot of existing software. We call the collection of tools and processes the “Surface Data System.”

Pavilion Lake 2009 Data Map

Pavilion Lake 2009 Data Map

We started with importing overlays of the bedrock geology from British Columbia Geological Survey (BCGS), as well as sonar bathymetry and sonar backscatter maps that show depth of the lake and structure of the bottom. These provide context for flight planning and for setting expectations for what we will find during the flights. In real time during the submarine flights, we get sub position every 5 seconds from the navigation computer on the chase boat. We use that to track the sub by periodically writing out updated KML files and automatically refreshing them in Google Earth as network links. A submarine icon shows the current location of the sub, and a compass rose shows bearings to indicate which way to go to reach the next waypoint. Google Earth also provides lots of measurement tools, annotation tools, and other built-in functions that we can use to annotate and analyze the map. All of this information is saved to a shared filesystem so that everyone at the camp has access to all of the same map data.

We have established an operations role on the support vessels which we call the “Science Stenographer”. That person’s job is to listen in on the voice loop and transcribe any significant observations in real time. Observations worth calling up to the surface immediately appear on the map as icons that you can click on to read the date, time, lat/long, and what was said.

My reflection in the monitor showing the stenography program

My reflection in the monitor showing the stenography program

In addition, the submarines are recording video continuously. The video recorder also has an “event” button that can mark the timestamp of a significant event on the video. In post-processing, we cross-correlate the timestamps to get position, and put a preview image and compressed video clip into the map as well.

These maps have been very useful for operations, for post-flight analysis, and for planning the next set of activities. After the flights are over, the science back room immediately has the flight track as-flown and georeferenced notes from the pilots. After some video post-processing (which takes some time simply due to the quantity of data: over 25 GB of video from each flight) the video clips and video stills are georeferenced and in the map. This information has been used to modify or create flight plans in real time. As an example, one morning Margarita identified interesting sampling locations during her flight. The post-flight map was immediately used to create a second flight plan to send Ricky to those locations for sample collection that same afternoon. It has also been interesting to see all of the flights and notes and images on one map simultaneously. The team realized before the end of the field season that there were areas of the lake that had not been covered and could plan accordingly.

Georeferenced notes attached to the flight tracks in Google Earth

Georeferenced notes attached to the flight tracks in Google Earth

Without this real time information procesing and integration, it would have been too late to go back and investigate those areas during the field season. Those flight plans would have to wait for another year. In a field setting with a lot of logistics overhead and a short duration for field work, understanding the big picture of operations quickly is a big time saver.

Georeferenced tree image in Pavilion Lake

Georeferenced tree image in Pavilion Lake

After the field season is over, the map data will be used by the team to analyse and correlate information from across the lake and across different flights to support their research, and to plan next year’s activities. All of the observations and information gathered this year bring up new questions and new hypotheses, and there is always more to study in this lake.

The images in this post show a few different views of map screens. There is also a KMZ file that you can download and open in Google Earth to see one of our flight plans and watch the time lapse animated flight track for the actual submarine positions flight as it was flown that day. Try setting the playback speed to the minimum for best results. Enjoy!

- Matt

DOWNLOAD THE KMZ FILE


Tuesday, July 14th, 2009

What Did You Do On Your Summer Vacation?

by Bree and Jen

This is a question that we ask our students on the first day of school in September. Boy, we have some interesting things to share on our first day of school. This summer, we travelled together to the Pavilion Lake Research Project to learn about the science that is being done here and how it can be incorporated into the classroom. During the year Bree and I are both immersed in the classroom, and most of the time we are teaching science. Today we have found ourselves writing a blog which neither of us have done before. We seem to be entertaining the people around us with our different ideas of how best to compose one of these, and we will find out if we get a gold star later. The crew here have been very welcoming and happy to share about what they do here. In fact, our first night here we were allowed to get inside one of the DeepWorker submersibles which are used during the scientist flight missions (we were still on dry land, but still very cool). Part of us being here at Pavilion Lake was to integrate teachers into the different activities that go on here, and integrate us they did.

From left, Bree Riddell, Ricky Arnold and Jen Stonehouse

From left, Bree Riddell, Ricky Arnold and Jen Stonehouse

So we are sure you are wondering what we were were able to do at the lake. Unfortunately we were not allowed to drive the subs but we had many other cool opportunities to be part of the team. We sat in on science meetings, pilot meetings, classified data from the submersibles, talked to scientists and astronauts, observed the launches of the submersibles and helped record data from the flights as a science stenographer. One of things that we did was classify the images returned from the underwater flights. During the flights images of what the pilots see is recorded on camera. The pilots see very cool things when on their mission – microbialites. After the mission this data then has to be classified. We looked at images to identified what was in the image – microbialites, algae, rocks, sediment, trash – oh my! This is something that can easily be transferred to the classroom. We classify every day just like the scientists (just on a different level). As the team here classifies these images for science and further understanding, students can also classify these images in the process of learning how to do science. Trust us (or read the rest of these blogs if you don’t), microbialites are very interesting!

How did we do with our first blog? Did we get a gold star?

-Jen and Bree


Friday, July 10th, 2009

Why do the microbialites have different shapes?

by Dawn Sumner
Different microbialites have different shapes.  Why?  How do we even answer this question?  One approach is to look at the building blocks of microbialites – the legos that stack together to make the structures.  Some microbialites have little tufts on them that make the surface rough.  These tufts are composed of the mineral calcite, which is intermixed with long, hair-like bacteria that come in two colors, pink and green.  The calcite makes the microbialite a rock, and the bacteria shape the tufts.  They are all lined up, pointing upward into the water like spiked hair.
These tufts are one of the building blocks for the microbialites.  How many there are, how they are arranged, and how fast they grow help shape the microbialites.  Lots of closely spaced tufts growing quickly create rough surfaces on the microbialites.  If the tufts grow slowly and there is lots of calcite, they only form small bumps.  If they are absent, the microbialites are smooth.  These smooth microbialites might need a different building block to form, one we haven’t identified yet.
The bacteria that form the tufts are photosynthetic – they use light to grow.  The tops of microbialites get more light than the sides do.  We can measure this, and it’s true in Pavilion Lake.  If tufts grow faster with more light and there is more light at the tops of the microbialites, we can predict that the tops might grow more quickly than the sides.  We haven’t measured this because the microbialites grow very slowly, and growth rates are very hard to measure.  However, the shapes of the microbialites suggest that they mostly grow upward.  Many of them have ridges on their sides pointing up or “chimneys” on top.  Thus, our prediction is consistent with the data we have so far.
Do we now understand why different microbialites have different shapes?  Nope!  The tuft building blocks that we’ve been analyzing should all behave about the same on near-by microbialites.  But the near-by microbialites aren’t all the same shape!  Maybe some of the differences are due to differences in growth rate, but I think there are probably more building blocks that we haven’t described yet.  To make a lego boat, you need different blocks than you do to make a lego submersible.  To make a dome-shaped microbialite, you might need different blocks than you do to make an “artichoke-shaped” microbialite.  We still have a lot to learn.

Different microbialites have different shapes.  Why?  How do we even answer this question? One approach is to look at the building blocks of microbialites – the legos that stack together to make the structures.  Some microbialites have little tufts on them that make the surface rough.  These tufts are composed of the mineral calcite, which is intermixed with long, hair-like bacteria that come in two colors, pink and green.  The calcite makes the microbialite a rock, and the bacteria shape the tufts.  They are all lined up, pointing upward into the water like spiked hair.

Close-up depiction of smooth and rough microbialite surfaces

Close-up depiction of smooth and rough microbialite surfaces

These tufts are one of the building blocks for the microbialites.  How many there are, how they are arranged, and how fast they grow help shape the microbialites.  Lots of closely spaced tufts growing quickly create rough surfaces on the microbialites.  If the tufts grow slowly and there is lots of calcite, they only form small bumps.  If they are absent, the microbialites are smooth.  These smooth microbialites might need a different building block to form, one we haven’t identified yet.

Smooth Microbialite Surface

Smooth Microbialite Surface

The bacteria that form the tufts are photosynthetic – they use light to grow.  The tops of microbialites get more light than the sides do.  We can measure this, and it’s true in Pavilion Lake.  If tufts grow faster with more light and there is more light at the tops of the microbialites, we can predict that the tops might grow more quickly than the sides.  We haven’t measured this because the microbialites grow very slowly, and growth rates are very hard to measure.  However, the shapes of the microbialites suggest that they mostly grow upward.  Many of them have ridges on their sides pointing up or “chimneys” on top.  Thus, our prediction is consistent with the data we have so far.

Closeup image of Microbialite

Closeup image of Microbialite

Do we now understand why different microbialites have different shapes?  Nope!  We still have a lot to learn, and many of the questions surrounding microbialite formation remain unanswered. The tuft building blocks that we’ve been analyzing should all behave about the same on near-by microbialites.  But the near-by microbialites aren’t all the same shape! Maybe some of the differences are due to differences in growth rate, but I think there are probably more building blocks that we haven’t described yet.  One thing to think about before I finish: To make a lego boat, you need different blocks than you do to make a lego submersible.  To make a dome-shaped microbialite, you might need different blocks than you do to make an “artichoke-shaped” microbialite.  This kind of thinking might help us solve one of the big mysteries of Pavilion Lake!

-Dawn


Thursday, July 9th, 2009

At Home in the Herms

by Bekah Shepard

If you want to make a bunch of Pavilion scientists excited, just mention “the herms”. I just did my second submersible flight through the herms and it was spectacular! Let me give you a little background, so that you can understand why the flight was such a treat:

A bioherm is a mound constructed by biological organisms. A classic example is a patch reef: a decimeter to meter scale mound that is built by corals, sponges, and other reef animals. You may not think of a mound of organisms as being very sturdy, but just as your body is capable of making hard bones, other organisms such as corals also make hard skeletons. When those skeletons start to pile up, you get a bioherm – a biological mound!

So, what in the world does this have to do with microbialites. Remember that microbialites are “organosedimentary structures”, meaning they are built up of minerals that are influenced by organisms. “Whoa!” I hear you cry, “Does that mean that a microbialite is a bioherm? Well, a single microbialite is not usually defined as a bioherm, but if you pile up enough microbialites, you do end up with a mound that is constructed by organisms! By that definition there are some places in Pavilion Lake where we observe large piles of microbialites that can be defined of bioherms.

Thinking about microbialite bioherms is something that some of us geologists do quite frequently. Long before the organisms that build coral reefs evolved, big bioherms and reef structures still existed. Rather than being built by corals, sponges, or even shells, the ancient bioherms and reefs were built out of microbialites! Can you imagine SCUBA diving or piloting a submersible around ancient microbialite bioherms and reefs! Boy, I wish I had a time machine. Since I don’t have a time machine, I do the next best thing. Can you guess what that is? Yup, I come here and study the microbialites in Pavilion Lake. The details we learn about the microbialites in Pavilion Lake will help us to understand the fossil record of ancient microbialite reefs. That will help us to understand how life evolved on early Earth! Crazy cool stuff!

Ok, back to the Pavilion Lake herms. Here is where we are going to get really confusing! The “herms” are not actually bioherms, hence the lack of the “bio”. The herms are an area of sediment mounds at the southern end of the central basin of the lake. Now I know you are screaming, “but if they aren’t bioherms, which are so cool, why are you crazy scientists so excited about them!” It turns out that even though the herms are just sediment mounds, they are covered by some of the most interesting microbialites in the lake. There are a lot of different microbialite morphologies crammed into a very small space. If you start at the bottom of any given herm, you can often see several distinct morphotypes just by looking up two meters of slope. Likewise, if you move around a herm several meters, you often can see changes in morphotype or surface texture. Those are rapid changes! I’m sure you are asking yourself why the microbialites would be changing that rapidly, and that is one of our big research questions this year! It may be that the mounds are a place of significant environmental variability. In other words, there may be interesting water flow patterns, light conditions, variations in sedimentation, etc. that are unique to the herms. Any of these variables may be influencing the morphology of the microbialites! In short, the herms are a really complicated place, and if you haven’t figured it out yet, the scientists as Pavilion Lake love to study really complicated and interested places!

-Bekah


Tuesday, July 7th, 2009

How to drive an underwater sports car in reverse?

by Allyson Brady

Tuesday was my first DeepWorker dive of the season. It was so great being back in the sub! I had almost forgotten how much fun it is to pilot and how amazing this lake is. The objective of my dive was to document transitions in the microbialites as you moved from deeper depths up slope to shallower depths and then back down again. We really want to get an understanding of how the microbialites vary within the lake and what types of lake characteristics (e.g. steepness of slope, sedimentation) might be associated with particular morphologies. This was a new type of dive as compared to the types we had planned last year and I wasn’t initially sure how easy it would be to back down the slope. Going up slope worked quite well, once I was back in pilot mode, remembering how best to combine my foot movements in order to minimize sediment disturbance.

It was really fascinating seeing how the microbialites change as you move into shallower water. You could really see that there were differences in the morphology as you changed depths. Transitions from columnar, smooth surface microbialites to rough, nodular looking ones were common. I also saw some huge microbialite mounds and microbialites growing on rocks and trees, very interesting for answering some of our science questions. Loads of algae were also visible in the shallower depths, everything from bright green filamentous algae to dark green material that resembles shag carpeting.

Screen Capture from the science stenographer showing Allyson's dive track on a map of the south basin in Pavilion Lake.

Screen Capture from the science stenographer showing Allyson's dive track on a map of the south basin in Pavilion Lake.

Once I was within 10 feet of the surface, the next objective was to move down slope along the same pathway capturing detailed video of the area. Hmmm…how do you back up the equivalent of a floating sports car with no rear view mirrors? As it turns out, very slowly and carefully actually does the job! After a few attempts, I think I was starting to get the hang of it and managed to get some quite nice video footage backing down the slope. It took a bit of practice but we’re all here to learn and that includes not just learning about the science, but learning about how best to explore our environment and collect the data that we use to answer our questions. At least I didn’t have to parallel park.

-Allyson


Monday, July 6th, 2009

flying a submersible… just like riding a bicycle

by Mike Gernhardt

This was my first Deepworker flight since last year, and I was pleased that flying the submersibles came back similar to skiing or riding a bicycle. We have been planning the science and operational metrics for this expedition for many months now, and it was both fun and exciting to get back into the water and execute the plan for real. I was constantly marveling at how cool it was to be seeing things that human eyes have never seen before, like exploring Mars or time warping back to pre-Cambrian oceans with today’s technology. It was great to be back working with the PLRP team, an extremely talented group that work together seamlessly to execute some very complex operations, which are helping us understand the optimal blending of science and operations in hostile environments as we get ready for the coming decades of planetary exploration.

The objectives of my dive today included contour mapping a part of the central basin that we have not seen before at 30 meters and 15 meters. It’s both challenging and fun to fly these contours. One of the more challenging aspects to learn was learning to fly only with my feet. The right foot is used to control direction and the left foot controls depth. With your left foot, push down with your right toe and you go forward, down with the right heel and you go backwards, twist right to turn right, twist left to turn left. With your right foot, push down with your left toe to dive, right heel to ascend. Then you blend all of those inputs to fly around various microbialite structures and contact lines, while simultaneously using your right hand to control the manipulator arm that positions the camera and your left had to operate the camera zoom and/ or the sonar, all the time while making observations and narrating into a voice recorder. If it sounds like a heavy workload, it is, and one of the things we are doing this year for the first time at PLRP is recording subjective human factors of the workload to compare with the quality of the objective and subjective data. By doing this, we will understand if factors like pilot fatigue play a role in the quality of science and exploration data obtained from the subs. In addition to be ground breaking science on earth, all of this contributes to the effort to help design the human factors of next generation of planetary surface exploration vehicles that optimize our ability to perform planetary exploration.

-Mike


Monday, July 6th, 2009

Anticipation, anticipation, anticipation!

by Bekah Shepard

The day before submersible operations is always rife with anticipation. Our whole team has been working all year to prepare for this mission, so now that we are so close to beginning, it can feel a bit torturous to wait one more day! However, there is lots of work and science to fill today. I spent some of my time today doing my first scuba dives of the field season. To make sure that everyone is safe, we do scuba checkout dives to practice our skills. After completing those, I headed off with a small team to the south basin of Pavilion Lake. We piled into a zodiac (a small, fast, inflatable boat) with our gear, and enjoyed the 10 minute ride south. Alex and Bernard jumped off, and swam over to place some sensors in one of the springs that we have discovered. Mike and I split off to investigate and collect samples of some of the microbial mats that inhabit the shallow waters of the lake.

Bekah preparing for a dive

Bekah preparing for a dive at Pavilion Lake

The mats are exciting for me because I am interested in the way some of the bacterial cells (specifically Cyanobacteria) move. The movement patterns of these single celled organisms can create complex shapes, or morphologies visible to the naked eye. Microbialites are “organosedimentary structures”, which just means that they are structures built out of microbial mats and minerals or sediments. The soft mats that I am studying are building complex structures, but they are not involving lots of minerals and sediments (they aren’t actually forming microbialites, so you might wonder about my interest). However, because the mats form complex morphologies, many of the bacterial behaviors that we observe in the soft mats will help us understand the formation and morphogenesis of other microbialites.

Studying these soft microbial mats involves lots of photographic documentation, as well as collecting samples for studying in the lab. We can actually grow these mats in the lab, and watch how the movement patterns work to build complex shapes. I work underwater with a pair of tweezers and carefully collect small pieces of mat into small plastic tubes. I carefully transport them back to the lab on shore, where we can begin our experiments. It doesn’t look very exciting underwater because I spend lots of time in the same place, but I actually really enjoy it! The mats are fascinating, and working underwater is very relaxing. Submersibles are fun, but sometimes it is nice to get your face right up close to what you are studying. They are both great ways to do science!

-Bekah


Monday, June 29th, 2009

Learning the Molecular Alphabet: DNA/RNA analysis at Pavilion Lake

by Zena Cardman

I am an undergraduate at the University of North Carolina, and this year I’ll be collecting microbialite samples, to figure out how the microscopic species living on the microbialites vary throughout the lake. Currently I’m working in a lab in the Department of Marine Science at UNC with Andreas Teske and Jen Biddle, where I study the genetic diversity of microbes living around a deep-sea hydrothermal vent site near Guaymas, Mexico. Even though Pavilion Lake is a very different environment, I’ll be using many of the same techniques to study the microbial life in Pavilion Lake as I do to study the microbes living in mud almost 3,000 meters below the surface of the ocean.

My background is in molecular biology, so to study the diversity of the microbialites’ microbes, Jen and I will extract their DNA and RNA, and then determine the sequences of particular genes that they code for. If you need a quick crash course in molecular biology: DNA is a molecule made up of four different types of subunits (called A, T, G and C for short), which are repeated and reordered to form a long chain. The particular order of these subunits stores the genetic information for all life forms, much like different combinations of letters spell out different words. Genes that are “switched on” write the words that code for RNA, a molecule which in turn gets translated into proteins. By looking at the differences between these sequences, we can gain some insight into what types of organisms they are, and how closely related they are. It would be very cool if we find differences in the microbes associated with different types of microbialites!

The DNA and RNA of a biological sample degrade quickly, so you have to preserve it if you want to be able to study it back home in your lab. Often samples are frozen at –80 degrees, or in liquid Nitrogen, in order to preserve their genetic information for future analysis. But it can be difficult to freeze samples like this, and safely ship them back to your lab when you are out doing field research. Instead, I’ll be using a salty solution to preserve the RNA in the microbialite samples I take. Field work is a fun challenge, because you have to be so prepared ahead of time! Everyone with the Pavilion Lake Research Project has been working hard for months already to make sure we’re ready for anything at the lake.

While there is barely a week left I leave for Pavilion Lake, I’m currently attending a course on astrobiology in Spain. The students are all young (mostly in graduate school, or recent PhDs), and it’s wonderful to witness the beginnings of collaborations between young scientists. I think astrobiology is especially conducive to collaboration, because it’s so interdisciplinary, and field projects like PLRP are no exception.

I can’t wait to see everyone at Pavilion Lake!

~Zena

Zena behind a mammoth-sized stack of clone libraries

Zena behind a mammoth-sized stack of clone libraries


Monday, June 22nd, 2009

Focusing on the details: 2009 DeepWorker Science Goals

by Margarita Marinova

Submersible tracks from the 2008 field season. Satellite image from Google Earth.

Submersible tracks from the 2008 field season. Satellite image from Google Earth.

As the field season nears, and everyone is busy finishing up all those details necessary to make our time in the field run smoothly, it’s important to refocus on our science goals for this year.

To quickly recap last year’s field season : our science pilots managed to explore most of the lake, giving a wealth of video footage and personal observations for the entire science team to analyze during the past year. You can see just how much of the lake we covered last year from all the submarine tracks! For the 2009 field season we have two main goals: to finish exploring some of the areas that we didn’t reach last year (you never know what’s hiding in the dark!); and to explore in detail the areas that we found to be particularly fascinating from our analysis of the 2008 data.

Following-up on the extensive exploration from last year, we are turning our attention to understanding some of the already identified features and trends, and using these to answer some big questions. In the lake we find microbialite structures that vary in shape and size. Some lake bottom areas are covered with the microbialites, while others have just sediment, or algae, or rocks from landslides. So why is there such a variation in what we find in the lake? How do the microbialites form? What sets their shape, their size, their distribution? And what can they tell us about the preservation of biosignatures – the fossils of microorganisms? These are hard questions that geobiologists have been trying to answer for decades, and we think that studying Pavilion Lake will contribute to the understanding of microbialites throughout Earth’s history. To tackle these questions, this field season we will be using the DeepWorkers to get detailed imaging and data at previously identified areas of interest.

Microbialite structures among the chara (algae). Are the microbialites using the chara as a base to grow on?

Microbialite structures among the chara (algae). Are the microbialites using the chara as a base to grow on?

Analysis of last year’s DeepWorker observations gave some surprising results, but also showed some possible trends that need to be investigated further. Last year we were surprised that the macro-morphology – the large-scale shape of the microbialite structures – was not strictly correlated with depth, like we thought it would be. So this year we hope that detailed observations needed to answer some smaller questions will ultimately help us answer our big questions. Some smaller questions we’re asking are: what are the microbialites growing on? Do they need a rock as a growing base, or algae, or do they just sit in the sediment? And does the growing medium have an effect on the shape or size of the microbialite? What about the small-scale structure (micro-morphology)? Microbialites with similar macro-morphologies can still be composed of differently shaped and sized components. The answers from all of these smaller questions will hopefully shed light on the bigger questions.

Every year we get some answers, and come up with even more questions. But that’s why this project is fascinating: Pavilion Lake is such a complex system! Little by little – by asking the right questions – the answers are coming together and we are starting to understand Pavilion Lake.

~Mars


Wednesday, March 25th, 2009

One microbialite, Two microbialites, Bulbous microbialite, Pointy microbialite

by Margarita Marinova

Welcome to the Pavilion Lake microbialite garden! To the right you will find the cauliflowers, straight ahead are the cauliflowers with chimneys, to the left we see the artichokes, and off in the deep end you can find some corals!

Cauliflowers with chimney structures

Cauliflowers with chimney structures

We didn’t plant the microbialite garden, but the Pavilion Lake Research Project team is there to study it. Microbialites are carbonate structures that form in water with the help of microorganisms. Commonly we see carbonates as limestone, but in certain special cases – often when life is involved – the carbonate precipitates to form some very unique structures. These microbialites are interesting because they can help us understand the types of structures that microorganisms form and the biological signatures they leave behind. We can then use this information to study similar structures from over 2.5 billion years ago. Microbialites are present in a number of lakes around the world, but what makes Pavilion Lake so special is the remarkable diversity! Not only is the lake full of microbialites, but the structures range in size, morphology (shape), and depth!

Artichoke structure

Artichoke structure

This is where our garden analogy comes in. As we explored the lake, we saw that the great diversity of microbialites fell into four general morphological categories: cauliflower, chimney, artichoke, and coral. From the four locations around the lake that we had decided to study intensely at the start of the project, these morphology types seemed to correlate with depth. We found the bulbous structures in the shallows, with chimneys, artichokes, and coral-like structures at increasing depths.

The microbialite structures are complex, and truly require a team with diverse skills and interests to study them. For me, the influence of physical factors on where and how the microbialites grow is incredibly interesting! Some specific questions that we are asking are: does the type of observed structure correlate with amount of light at that location? Is the mineral composition of the microbialites different between structures? Does the depth or the type of material on the lake bottom determine what type of microbialite grows? Studying the effects of light and temperature tell us whether biology is involved, as photosynthesis and therefore rate of growth depend on these parameters. Conversely, a relationship between mineralogy and morphology could mean a more abiological and chemical control on the shapes of the microbialites. One way of answering these questions is by placing sensors on the lake bottom – near microbialite structures – and recording the environmental conditions that the microbialites feel throughout the year. To assess environmental conditions we must collect years of data to be confident in the trends: for example, just because one year is cloudy and cold doesn’t mean this is always the case!

Cauliflower structures

Cauliflower structures

Another important approach that was started last year has been the use of the Deepworker submersibles. With these submarines we have been able to take high definition video of everything that the pilot sees: truly exploring the lake bottom without the constraints of scuba diving. We have used the video collected by the subs to map the lake bottom, that meant looking at more than 70,000 images, but it was well worth it! The mapping allowed us to really understand the distribution of microbialites over the entire lake and be able to more confidently say if morphology type correlates with factors like depth or lake bottom material. We certainly were in for some surprises when it came to the distribution of morphologies! For example we found that artichoke structures are distributed over a much larger range of depths than we had previously thought, and this coming summer we will be testing the hypothesis that the microbialites need a hard surface, like a rock, to start growing.

Coral-like structures in the deep end

Coral-like structures in the deep end

There is a lot more work to be done in understanding these microbialite structures. Pavilion Lake keeps us coming back with its fascinating science questions and enchanting structures: the depths of the lake are a window to the past, yet rooted in the present. Year after year we use these structures as a key to understanding the oldest life, the smallest organisms, and the most wondrous lake in beautiful British Columbia. What’s not to love?

:-) Mars